dpp-4 inhibitor screening test assay kit  (Cayman Chemical)

Journal: Biomedicines

Article Title: Minimum Effective Dose of DPP-4 Inhibitors for Treating Early Stages of Diabetic Retinopathy in an Experimental Model

doi: 10.3390/biomedicines10020465

Figure Lengend Snippet: DPP-4 protein abundance. ( A , C ) Comparison of retinal DPP-4 immunofluorescence reactivity (red) among representative samples of each experimental group. DPP-4 relative fluorescence intensity is presented alone and merged with Hoechst nuclei staining (blue). ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer. Scale bars, 20 µm. ( B , D ) Quantification of the DPP-4 immunofluorescence intensity. n = 4 mice per group and 5 retinal sections for each retina. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Then, sections were blocked with blocking solution (10% normal goat serum (31873, ThermoFisher Scientific, Waltham, MA, USA) + 1% BSA (A3059-100G, Millipore Sigma, Burlington, MA, USA) in PBS) for 1 h at room temperature, and afterwards, they were incubated overnight at 4 °C with the anti-GFAP (rabbit monoclonal; 1:500; ab7260; Abcam, Cambridge, UK) or the anti-DPP-4 (rabbit polyclonal; 1:200; ab28340; Abcam, Cambridge, UK) primary antibodies.

Techniques: Immunofluorescence, Fluorescence, Staining

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Dipeptidyl peptidase-4 is associated with myogenesis in patients with adolescent idiopathic scoliosis possibly via mediation of insulin sensitivity

doi: 10.1186/s13018-022-02978-w

Figure Lengend Snippet: Serum DPP-4 level in AIS patients and healthy controls. The serum DPP-4 activity was remarkably lower in AIS patients than in the healthy controls. * p < 0.05 using two-tailed Student’s t -test. Data are presented as mean ± standard deviation

Article Snippet: Primary antibodies were listed as follows: DPP-4 (1:2000; ab28340, Abcam), Ras (1:5000; ab52939, Abcam), p-ERK1/2 (1:5000; ab201015, Abcam), p-mTOR (1:1000; #5536, Cell signaling technology), p-AKT (1:2000, #4060, Cell signaling technology) and GAPDH (1:2000; #5174, Cell signaling technology).

Techniques: Activity Assay, Two Tailed Test, Standard Deviation

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Dipeptidyl peptidase-4 is associated with myogenesis in patients with adolescent idiopathic scoliosis possibly via mediation of insulin sensitivity

doi: 10.1186/s13018-022-02978-w

Figure Lengend Snippet: DPP-4 gene and protein expression in muscle tissue isolated from LDH patients and AIS patients. A , B Relative expression of DPP4 and STAT1 was significantly decreased in patients ( n = 45) as compared with that of the controls ( n = 30). C mRNA expression level of DPP4 gene were strongly correlated with STAT1 mRNA expression level ( r = 0.41, p < 0.05). Pearson’s correlation analysis was used to determine the correlation. D , E Western blot showed DPP-4 protein expression in muscle was significantly higher in controls ( n = 4) as compared with AIS patients ( n = 4). * p < 0.05, two-tailed Student’s t test. Data are presented as mean ± standard deviation

Article Snippet: Primary antibodies were listed as follows: DPP-4 (1:2000; ab28340, Abcam), Ras (1:5000; ab52939, Abcam), p-ERK1/2 (1:5000; ab201015, Abcam), p-mTOR (1:1000; #5536, Cell signaling technology), p-AKT (1:2000, #4060, Cell signaling technology) and GAPDH (1:2000; #5174, Cell signaling technology).

Techniques: Expressing, Isolation, Western Blot, Two Tailed Test, Standard Deviation

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Dipeptidyl peptidase-4 is associated with myogenesis in patients with adolescent idiopathic scoliosis possibly via mediation of insulin sensitivity

doi: 10.1186/s13018-022-02978-w

Figure Lengend Snippet: Impact of treatment with glucose and insulin on DPP-4 expression. Cells were treated with glucose (10 mM) and insulin (0.5 nM) for 24 h. A The mRNA expression of DPP-4 was significantly increased in the treated control cell as compared with the untreated control cells ( n = 3/group). B The DPP-4 protein level was significantly increased in the treated control cells as compared with the untreated control cells ( n = 3/group). No significant difference of DPP4 mRNA or protein expression level was observed between the treated and untreated cells in AIS. * p < 0.05, two-tailed Student’s t test, two-tailed Student’s t test. Data are presented as mean ± standard deviation

Article Snippet: Primary antibodies were listed as follows: DPP-4 (1:2000; ab28340, Abcam), Ras (1:5000; ab52939, Abcam), p-ERK1/2 (1:5000; ab201015, Abcam), p-mTOR (1:1000; #5536, Cell signaling technology), p-AKT (1:2000, #4060, Cell signaling technology) and GAPDH (1:2000; #5174, Cell signaling technology).

Techniques: Expressing, Two Tailed Test, Standard Deviation

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Dipeptidyl peptidase-4 is associated with myogenesis in patients with adolescent idiopathic scoliosis possibly via mediation of insulin sensitivity

doi: 10.1186/s13018-022-02978-w

Figure Lengend Snippet: Relationship between DPP-4 and clinical features of AIS. DPP4 gene expression in muscle tissue was measured by RT-qPCR and analyzed with the 2 −ΔΔCT method ( n = 45). A There was a significant correlation between the expression level of DPP-4 and BMI in AIS patients. B There was no significant correlation between DPP4 expression and curve severity. C No significant correlation was observed between DPP-4 expression and BMD

Article Snippet: Primary antibodies were listed as follows: DPP-4 (1:2000; ab28340, Abcam), Ras (1:5000; ab52939, Abcam), p-ERK1/2 (1:5000; ab201015, Abcam), p-mTOR (1:1000; #5536, Cell signaling technology), p-AKT (1:2000, #4060, Cell signaling technology) and GAPDH (1:2000; #5174, Cell signaling technology).

Techniques: Expressing, Quantitative RT-PCR

Journal: American Journal of Translational Research

Article Title: Sitagliptin protects against hypoxia/reoxygenation (H/R)-induced cardiac microvascular endothelial cell injury

doi:

Figure Lengend Snippet: Hypoxia/reoxygenation (H/R) increased the expression of DPP-4 in primary cardiac microvascular endothelial cells (CMECs). Hypoxia/reoxygenation experiments were performed by putting cells in an air-tight chamber with pure N2 followed by incubation at 37°C for 6 h. Cells were then cultured with normal medium in a normoxic incubator (95% air plus 5% CO2) for 12 h for reoxygenation. A. Expression of DPP-4 at the mRNA levels was determined by real time PCR analysis; B. Expression of DPP-4 at the protein levels was determined by western blot analysis (*, P<0.01 vs. the control group, n=6).

Article Snippet: The following antibodies were used in this study: DPP-4 (1:1000, #ab28340, Abcam, USA); NOX-4 (1:2000, ab133303, Abcam, USA); p-p38 (1:1000, #9216, Cell Signaling Technology, USA); p38 (1:3000, #8690, Cell Signaling Technology, USA); p65 (1:2000, #9460, Cell signaling technology, USA); Lamin B1 (1:5000, #13435, Cell Signaling Technology, USA); β-actin (1:10000, #3700, Cell Signaling Technology, USA); anti-rabbit IgG, HRP-linked antibody (1:2000, #7074, Cell Signaling Technology, USA); anti-mouse IgG, HRP-linked antibody (1:2000, #7076, Cell Signaling Technology, USA).

Techniques: Expressing, Incubation, Cell Culture, Real-time Polymerase Chain Reaction, Western Blot